Functional diversification of Arabidopsis SEC1-related SM proteins in cytokinetic and secretory membrane fusion
نویسندگان
چکیده
منابع مشابه
Selective Activation of Cognate SNAREpins by Sec1/Munc18 (SM) Proteins
Full-length mouse VAMP2-His 6 (pTW2) and the t-SNARE complex between mouse His 6-SNAP-25 and rat syntaxin 1A (pTW34) were expressed and purified as previously described (Melia et al., 2002; Weber et al., 2000). Rat syntaxin 4 and SNAP-23 expression vectors were generated in a similar way as neuronal SNAREs. The proteins were eluted into an OG buffer (25mM HEPES/KOH, pH 7.4, 400 mM KCl, 10% glyc...
متن کاملMembrane fusion: grappling with SNARE and SM proteins.
The two universally required components of the intracellular membrane fusion machinery, SNARE and SM (Sec1/Munc18-like) proteins, play complementary roles in fusion. Vesicular and target membrane-localized SNARE proteins zipper up into an alpha-helical bundle that pulls the two membranes tightly together to exert the force required for fusion. SM proteins, shaped like clasps, bind to trans-SNAR...
متن کاملSec1/Munc18 Proteins Mediators of Membrane Fusion Moving to Center Stage
sive apoptotic degeneration occurs, but only becomes Synaptic transmission is mediated by exocytosis of neuapparent after initial synaptogenesis, thus suggesting rotransmitter-filled synapic vesicles. In recent years, we that synaptic activity is not needed for the initial estabhave learned that many of the proteins involved in exolishment of synaptic connectivity (Verhage et al., 2000). cytosi...
متن کاملfunctional study of p0 proteins of two cereal yellow dwarf viruses (cydv-rpv and cydv-rps) and identification of their cellular partner
نقش سرکوبگری پروتئین p0 در دو پولروویروس کوتولگی زردی غلات cydv-rpv) و (cydv-rps، متفاوت در شدت بیماریزایی، مورد مطالعه قرار گرفت. نتایج نشان داد که هر دو پروتئین p0 p0cy-rpv) و (p0cy-rps قادر به سرکوب خاموشی آر ان ای ایجاد شده توسط ترادف های تراژن سنس و تکرار معکوس در n. benthamiana هستند. نشان داده شد که هر دو پروتئین p0 می توانند تخریب پروتئین argonaute-1 را تسهیل کنند. علاوه بر این، تمایل م...
Evolutionary diversification of the Sm family of RNA-associated proteins.
The Sm family of proteins is closely associated with RNA metabolism throughout all life. These proteins form homomorphic and heteromorphic rings consisting of six or seven subunits with a characteristic central pore, the presence of which is critical for binding U-rich regions of single-stranded RNA. Eubacteria and Archaea typically carry one or two forms of Sm proteins and assemble one homomor...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2018
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1722611115